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Biocolor 硫酸氨基聚糖檢測試劑盒(Blyscan™ Glycosaminoglycan As

簡要描述:采用常規生化方法,通過標準曲線和比色,可定量分析總硫酸化多糖(氨基聚糖和蛋白聚糖)的含量,亦可測定O端和N端硫酸氨基多糖的比值。主要采用的是Blyscan染料(Biocolor公司的產品),它可以特異性的與氨基聚糖和蛋白聚糖上的硫酸多糖組分結合。

  • 產品型號:B1000
  • 廠商性質:代理商
  • 更新時間:2016-03-28
  • 訪  問  量:1133

產品介紹

Biocolor 硫酸氨基聚糖檢測試劑盒 
Biocolor Blyscan™ Glycosaminoglycan Assay

 
    采用常規生化方法,通過標準曲線和比色,可定量分析總硫酸化多糖(氨基聚糖和蛋白聚糖)的含量,亦可測定O端和N端硫酸氨基多糖的比值。主要采用的是Blyscan染料(Biocolor公司的產品),它可以特異性的與氨基聚糖和蛋白聚糖上的硫酸多糖組分結合。該方法不適于檢測已經降解的硫酸化雙糖片段。
    該試劑盒可檢測廣泛的蛋白多糖,可檢測的氨基多糖包括硫酸軟骨素、硫酸角質素、硫酸皮膚素、硫酸肝素,不適合檢測包含海藻酸的樣品,因為它們含有糖醛酸。該試劑盒也不能對透明質酸定量,但透明質酸并不干擾對硫酸化氨基多糖的檢測。細胞培養基中20%的胎牛血清也不會干擾實驗。該方法檢測靈敏度為5ug,所需時間為1小時。
 
檢測樣本:可來自軟骨組織、動脈組織、肺組織、皮膚組織、腫瘤組織、細胞核組織提取物、細胞培養液、羊水和尿液
 
實驗操作 
1. 繪制標準曲線
2. 待測樣品的處理:
取50ul待測樣品進行實驗。如果反應物在656nm初的吸收峰大于1.5,則對樣品進行稀釋,待測樣樣品濃度落入標準曲線中段時,準確度較高。
3. 硫酸氨基聚糖—染料復合物的獲得
加1.0ml的Blyscan染料溶液到待測樣本中,充分混勻,待不溶物出現,離心,棄上清,則獲得紫色的硫酸氨基聚糖—染料復合物
4. 硫酸氨基聚糖—染料復合物中染料的釋放
加入1.0ml的染料釋放劑,充分混勻。溶解5min以上,使染料充分釋放
5. 測定
用分光光度計于波長656nm處測定,或使用colorimeter紅色濾光片測定
 
產品訂購

   Product Code: B1000     110 assays 
   Product Code: B3000     440 assays

 

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